A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea
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چکیده
منابع مشابه
A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea
BACKGROUND Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment sam...
متن کاملNovel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment.
Metagenomic cloning is a powerful tool for the discovery of novel genes and biocatalysts from environmental microorganisms. Based on activity screening of a marine sediment microbial metagenomic library, a total of 19 fosmid clones showing lipolytic activity were identified. After subcloning, 15 different lipolytic genes were obtained; their encoded proteins showed 32-68% amino acid identity wi...
متن کاملA Novel Cold Active Esterase from a Deep Sea Sponge Stelletta normani Metagenomic Library
1 School of Microbiology, University College Cork, Cork, Ireland, Department of Microbiology, School of Life Sciences, Pondicherry University, Pondicherry, India, Department of Food Science and Technology, Pondicherry University, Pondicherry, India, 4 Biomerit Research Centre, University College Cork, Cork, Ireland, 5 School of Biomedical Sciences, Curtin Health Innovation Research Institute, C...
متن کاملIdentification of novel esterase from metagenomic library of Yangtze river.
A metagenomic library of surface-water microbes from the Yangtze River in China was constructed, and a novel esterase, designated as EstY, was isolated and characterized. EstY had 423 amino acids with an estimated molecular mass of 44 kDa and pI of 7.28. It hydrolyzed various pnitrophenyl esters (acetate, butyrate, caprate, caprylate, laurate, myristate, and palmitate) and its best substrate wa...
متن کاملIdentification and Characterization of a Novel Salt-Tolerant Esterase from the Deep-Sea Sediment of the South China Sea
Marine esterases play an important role in marine organic carbon degradation and cycling. Halotolerant esterases from the sea may have good potentials in industrial processes requiring high salts. Although a large number of marine esterases have been characterized, reports on halotolerant esterases are only a few. Here, a fosmid library containing 7,200 clones was constructed from a deep-sea se...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2011
ISSN: 1475-2859
DOI: 10.1186/1475-2859-10-95